Vascular Endothelial Growth Factor Receptor 2, phosphorylated (Tyr1214) (VEGF R2, Flk1, KDR, Receptor Tyrosine Kinase)

Vascular Endothelial Growth Factor Receptor 2 (VEGFR2, also known as KDR/FLK-1) is a ~200kD member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. As with other growth factor receptors, upon ligand binding, VEGFR2 dimerizes and is autophosphorylated on multiple tyrosine residues. These phosphotyrosine sites are involved in regulation of intrinsic tyrosine kinase activity or serve as binding sites for SH2 domains and phosphotyrosine binding (PTB) domains of downstream signaling proteins. Phosphorylation of tyrosine 1214 is reported to play a critical role in regulation of receptor autophosphorylation and stimulation of intrinsic tyrosine kinase catalytic activity. \n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.35-1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nNIH3T3 cells transfected with full-length, wild-type human VEGFR2 and Porcine Aortic Endothelial (PAE) cells transfected with a chimeric receptor consisting of the extracellular domain of the CSF-1 receptor coupled to the transmembrane and cytoplasmic domains of the mouse VEGFR2.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

V2110-19G

Size

10 Blots

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Meyer, R.D., et al. (2002) The presence of a single tyrosine residue at the carboxyl domain of vascular endothelial growth factor receptor-2/FLK-1 regulates its autophosphorylation and activation of signaling molecules. J. Biol. Chem. 277(30):27081-27087. 2. Habeck, H., et al. (2002) Analysis of a zebrafish VEGF receptor mutant reveals specific disruption of angiogenesis. Curr. Biol. 12(16):1405-1412. 3. Rahimi, N., et al. (2000) Receptor chimeras indicate that the vascular endothelial growth factor receptor-1 (VEGFR-1) modulates mitogenic activity of VEGFR-2 in endothelial cells. J. Biol. Chem. 275(22):16986-16992. 4. Shalaby, F., et al. (1995) Failure of blood-island formation and vasculogenesis in Flk-1-deficient mice. Nature 376(6535):62-66.

Additional Info

Recognizes human and mouse VEGFR2 when phosphorylated at Tyr1214. Species sequence homology: Rat (100%).