Vascular Endothelial Growth Factor (VEGF) (BSA & Azide Free)

VEGF (vascular endothelial growth factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF189 and VEGF206) remain cell associated.\n\nApplications:\nSuitable for use in Western Blot. Not suitable for Neutralization. Other applications have not been tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml for 2 hours at RT. Reducing conditions only.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nVEGF165 recombinant protein\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

V2110-12X

Size

100ug

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See V2110-12.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

1. Boocock, C.A., et al., Journal of the National Cancer Institute 87(7): 506-516 (1995). 2. Brown, L.F., et al., Human Pathology 26(1): 86-91 (1995).

Additional Info

Recognizes the human Vascular Endothelial Growth Factor (VEGF) at ~19-22kD. Does not neutralize the bioactivity of VEGF.