Vascular Endothelial Growth Inhibitor (VEGI)

Vascular endothelial growth inhibitor (VEGI) belongs to the human tumor necrosis factor (TNF) family and acts as a multifunctional cytokine. VEGI is expressed predominantly in endothelial cells and is an endothelial cell-specific negative regulator of angiogenesis. Human VEGI protein contains 174 amino acids. The calculated molecular weight is approximately 20kD. An experiment examining in vitro transcription and translation involving the use of a cDNA clone as a template revealed a similar molecular weight of approximately 22kD. Hydrophobicity analysis predicts a 13 amino acid hydrophobic region that follows the C-terminal segment of 12 residues; this is typical of a type II transmembrane protein with the C-terminus on the extracellular surface, a single transmembrane domain, and a short cytoplasmic tail. Northern blots reveal that VEGI transcripts have been observed in the endothelial cells of placenta, lung, kidney, skeletal muscle, pancreas, spleen, prostate, small intestine, and colon. \n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 0.1-1.0ug/ml \nWestern Blot: 1-2ug/ml \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

V2121-35

Size

100ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Haridas V, et al. VEGI, a new member of the TNF family, activates nuclear factor-kappa B and c-Jun N-terminal kinase and modulates cell growth. Oncogene 18(47): 6496-6504, (1999). 2. Zhai Y, et al. Inhibition of angiogenesis and breast cancer xenograft tumor growth by VEGI, a novel cytokine of the TNF superfamily. Int J Cancer. 82(1): 131-136, (1999). 3. Zhai Y, et al. VEGI, a novel cytokine of the tumor necrosis factor family, is an angiogenesis inhibitor that suppresses the growth of colon carcinomas in vivo. FASEB J. 13(1): 181-189, (1999).

Additional Info

Recognizes human VEGI at~20kD. Reacts with human PC-3 prostate adenocarcinoma cell lysates.