Vasoactive Intestinal Peptide (VIP, MGC13587, PHM27)

Vasoactive intestinal peptide (VIP) is a 28 amino acid peptide belonging to the secretin-glucagon family of peptide hormones. Its sequence is identical in man, ox, porcine and rat. VIP is widely distributed in the central and peripheral nervous systems, acting as a neurotransmitter or neuromodulator. Peripherally it is involved in vaso- and bronchodilatation, smooth muscle relaxation and stimulation of secretion. Ectopic endocrine tumors secreting VIP (VIPomas) give rise to raised plasma levels of VIP and are associated with watery diarrhea, hypokalemia and achlorhydria syndrome.\nVIP shows high sequence homology with peptide histidine-methionine (PHM) in man or peptide histidine-serine (PHI) in porcine or rat, present in the same precursor, and with the neuropeptide pituitary adenylate cyclase activating polypeptide (PACAP).\n\nApplications: \nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:500; VIP coated directly at 2ug/ml. For glutaraldehyde-linked VIP-ovalbumin (molar ratio 4:1) coated at 2ug/ml, the dilution is 1:16,000.\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nSp2mIL6 myeloma cells with spleen cells from NMRI x Balb/c mice. \n\nCulture Medium:\nRPMI 1640 with 10% fetal calf serum\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts