Vasodilator Stimulated Phosphoprotein, phosphorylated (Ser157) (VASP)

VASP (vasodilator stimulated phosphoprotein) plays an important role in ANF/NO/cGMP Protein kinase and cAMP/cAMP Protein kinase signalling pathways. VASP is expressed in almost all human and animal cell lines; particularly high concentrations are found in thrombocytes, vascular smooth muscle cells and fibroblasts. In cultured cells VASP is associated with focal contacts, cell-cell-contacts, microfilaments and dynamic membrane regions such as the leading edge. In vitro binding data show that VASP binds to profilin, zyxin, vinculin, and the Listeria spp. surface protein ActA. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament formation. Ser 157 is a site preferred by cAMP-dependent protein kinase (PKA). The antibody is able to monitor the phosphorylation state of VASP serine 157 as well as PKA activity.\n\nApplications: \nSuitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.05ug/ml\nWestern Blot: 0.5ug/ml for HRPO/ECL detection. Casein/Tween 20 based blocking and blot incubations are recommended.\nImmunoprecipitation: 1-10ug per 10e6 pervandate-treated A431 cells\nImmunocytochemistry: 1-10ug/ml. May tolerate 0.5% formaldehyde fixation.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

V2118-02E

Size

50ug

Applications

ELISA, ICC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, PEG, sucrose, 0.09% sodium azide.

P Type

Mab

Purity

Purified by size exclusion chromatography.

Isotype

IgG1

Additional Info

Recognizes Vasodilator Stimulated Phosphoprotein at ~46/50kD when phosphorylated at Ser157. Does not crossreact with the non-phosphorylated form of VASP or with unrealated serine-phosphorylated proteins. Species Crossreactivity: human, mouse