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Vasodilator Stimulated Phosphoprotein, phosphorylated (Ser238) (VASP)

Vasodilator Stimulated Phosphoprotein, phosphorylated (Ser238) (VASP)

Cat no: V2118-01P

Supplier: United States Biological
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VASP is a novel phosphoprotein associated with microfilaments, focal adhesions and highly dynamic membranes not only in human platelets but also in a wide variety of other cells and tissues. VASP was originally discovered, purified and characterized as a protein which is phosphorylated in response to both cAMP and cGMP elevating vasodilators and platelet inhibitors mediated by cAMP and cGMPdependentprotein kinases (cAK, cGK). The protein is involved in signal transduction and cellular motility. Morever as the protein contains both actin binding and actin monomer-nucleating capabilities, the molecule is also of particular interest for reorganization of the cytoskeleton. Human VASP gene is localized on chromosomal region 19q13.2-q13.3. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:1000 Immunohistochemistry (paraffin): 1:50-1:100 Optimal dilutions to be determined by the researcher. Positive Control: NIH/3T3 cells Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: V2118-01P
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 50ug
Form: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
Alternative names: Vasodilator-stimulated Phosphoprotein
References: 1. Matthias, R. et al. The EMBO Journal. 14:1583-1589 (1995). 2. Peter R. et al. The FASEB Journal. 21:2613-2621 (2007).
Additional info: Recognizes human VASP when phosphorylated at Ser238. Species Crossreactivity: mouse and rat.

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