Vasodilator Stimulated Phosphoprotein, phosphorylated (Ser239) (VASP) (Biotin)

VASP (vasodilator stimulated phosphoprotein) plays an important role in ANF/NO/cGMP Protein kinase and cAMP/cAMP Protein kinase signalling pathways. VASP is expressed in almost all human and animal cell lines; particularly high concentrations are found in thrombocytes, vascular smooth muscle cells and fibroblasts. In cultured cells VASP is associated with focal contacts, cell-cell-contacts, microfilaments and dynamic membrane regions such as the leading edge. In vitro binding data show that VASP binds to profilin, zyxin, vinculin, and the Listeria spp. surface protein ActA. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament formation. Ser 157 is a site preferred by cAMP-dependent protein kinase (PKA). \n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunocytochemistry, Flow Cytometry and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions: \nELISA: 0.05ug/ml, as reported by an independent laboratory.\nWestern Blot: 0.5-2ug/ml detected phosphorylated VASP in RIPA lysates from CTLL cells. CTLL cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with V2118 (0.5ug/ml). Proteins were visualized using a Gt xMo IgG (HRP)* and a chemiluminescence detection system.\nImmunocytochemistry: An independent laboratory has reported that 3ug/ml antibody showed positive immunostaining in SNP or 8pCPT-cGMP-treated human platelets, ?xed with 4% paraformaldehyde and permeabilized with 0.2% Triton-X. \nFlow Cytometry: 2.4ug/ml using ?xed and permeabilized human platelets, as reported by an independent laboratory. \nImmunoprecipitation: Immunoprecipitates phosphorylated VASP from vanadate-treated A431 cells, as reported by an independent laboratory.\nOptimal dilutions to be determined by the researcher.\n\n*Recommended secondary antibodies:\n I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-14C: IgG, Fc (HRP) Pab Gt xMo \nI1904-19C: IgG, F(ab

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SPECIFICATIONS

Catalog Number

V2118-03B

Size

50ug

Applications

ELISA, FC, ICC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Conjugates

Biotin

Form

Supplied as a liquid in PBS.

P Type

Mab

Purity

Puri?ed by thiophilic adsorption and size exclusion chromatography.

Isotype

IgG1,k

References

1. Analysis and Regulation of Vasodilator-stimulated Phosphoprotein Serine 239 Phosphorylation in Vitro and in Intact Cells Using a Phosphospecific Monoclonal Antibody: A. Smolenski, et al.; J. Biol. Chem. 273, 20029 (1998)\n2. Flow cytometry analysis of intracell. VASP phosphorylation for the assessment of activating and inhibitory signal transduct. pathways in human platelets-definition & detect. of ticlopidine/clopidogrel: U.R. Schwarz, et al.; Thromb. Haemost. 82, 1145 (1999) \n3. Effects of In Vivo Nitroglycerin Treatment on Activity and Expression of the Guanylyl Cyclase and cGMP-Dependent Protein Kinase and Their Downstream Target Vasodilator-Simulated Phosphoprotein in Aorta: A. M

Additional Info

Recognizes human phosphorylated VASP, Mr 50kD. \nSpecies Crossreactivity: mouse