Vasodilator Stimulated Phosphoprotein, phosphorylated (Ser239) (VASP)

VASP (vasodilator stimulated phosphoprotein) plays an important role in ANF / NO / cGMP Protein kinase and cAMP / cAMP Protein kinase signalling pathways. VASP is expressed in almost all human and animal cell lines; particularly high concentrations are found in thrombocytes, vascular smooth muscle cells and fibroblasts. In cultured cells VASP is associated with focal contacts, cell-cell-contacts, microfilaments and dynamic membrane regions such as the leading edge. In vitro binding data show that VASP binds to profilin, zyxin, vinculin, and the Listeria spp. surface protein ActA. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament formation. \nSer239 is a phosphorylation site preferred by cGMP-dependent protein kinase (PKG), but also used by cAMP-dependent protein kinase (PKA). Because the antibody does not crossreact with the non-phosphorylated form of VASP, nor with unrelated serine-phosphorylated proteins; V2118-03 is able to monitor the phosphorylation state of VASP serine 239 as well as PKA/PKG activity.\n\nApplications:\nSuitable for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunoprecipitation and Western Blot. Other applications have not been tested. \n\nRecommended Dilution:\nELISA: 0.05ug/ml\nImmunocytochemistry: 1-10ug/ml. May tolerate 0.5% formaldehyde fixation.\nImmunoprecipitation: 1-10ug/10e6 vanadate treated A431 cells.\nWestern Blot: 1ug/ml for AP/BCIP/NBT (MTT) detection, 0.1-1ug/ml for HRPO/ECL detection.\nOptimal dilutions to be determined by researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

V2118-03

Size

50ug

Applications

ELISA, FC, ICC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, PEG and sucrose.

P Type

Mab

Purity

Purified by thiophilic adsorption and size exclusion chromatography.

Isotype

IgG1,k

References

1. Smolenski, A., et al., J. Biol. Chem. 273: 20,029 (1998). 2. Schwarz, U.R., et al., Thromb. Haemost. 82: 1145 (1999). 3. M

Additional Info

Recognizes human VASP phosphorylated at Ser239. Does not crossreact with the non-phosphorylated form of VASP, nor with unrelated serine-phosphorylated proteins. Species Crossreactivity: Mouse.