A unique immunoperoxidase procedure for localizing a variety of histologically significant antigens and other markers was originally introduced by Su-Ming Hsu and his associates.
* This patented procedure employs biotinylated antibody and a preformed Avidin: Biotinylated enzyme Complex and has been termed the “ABC” technique.
Because avidin has such an extraordinarily high affinity for biotin (over one million times higher than antibody for most antigens), the binding of avidin to biotin is essentially irreversible.