Vimentin is the major subunit protein of the intermediate filaments of mesenchumal cells, and has a molecular weight of 57kD. Immunohistochemical staining for Vimentin is thus characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of malignant tumors, generally used with a panel of other antibodies including those recognizing cytokeratins, lymphoid markers, S100, desmin and neurofilaments. The antibody stains the 57kD vimentin band in immunoblots performed on a lysate of normal human lymphocytes. It does not stain mouse or rat vimentin.
Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry (paraffin): 1:50-1:100
Immunohistochemistry (frozen): 1:300, in PBS.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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