VPS35 (Vacuolar Protein Sorting-associated Protein 35, hVPS35, Maternal-embryonic 3, Vesicle Protein Sorting 35, MEM3, TCCCTA00141)

Cat no: V0650-24C

Supplier: United States Biological

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Description
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VPS35, otherwise known as MEM3, is a ubiquitously expressed member of the vacuolar protein sorting (VPS) family, and vital component of the multimeric retromer complex, involved in the retrieval of lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans Golgi network. Applications: Suitable for use in ELISA, Immunohistochemistry, Immunofluorescence and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:32,000 Western Blot: 0.1-1ug/ml, observed in human brain cerebellum lysates on ~85kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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Catalogue number:	V0650-24C
Reactivities:	Human, Mouse, Rat, Bovine
Hosts:	Goat
Applications:	ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Size:	100ug
Form:	Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
P type:	Pab
Purity:	Purified by immunoaffinity chromatography.
References:	1. Scott KL, Kabbarah O, Liang MC, Ivanova E, Anagnostou V, Wu J, Dhakal S, Wu M, Chen S, Feinberg T, Huang J, Saci A, Widlund HR, Fisher DE, Xiao Y, Rimm DL, Protopopov A, Wong KK, Chin L. GOLPH3 modulates mTOR signalling and rapamycin sensitivity in cancer. Nature. 2009 Jun25;459(7250):1085-90. 2. Gokool S, Tattersall D, Seaman MN. EHD1 interacts with retromer to stabilize SNX1 tubules and facilitate endosome-to-Golgi retrieval. Traffic. 2007 Dec;8(12):1873-86. 3. Nielsen MS, Gustafsen C, Madsen P, Nyengaard JR, Hermey G, Bakke O, Mari M, Schu P, Pohlmann R, Dennes A, Petersen CM. Sorting by the cytoplasmic domain of the amyloid precursor protein binding receptor SorLA. Mol Cell Biol. 2007 Oct;27(19):6842-51.
Additional info:	Recognizes human VPS35. Species sequence homology: Bovine, mouse and rat.

VPS35 (Vacuolar Protein Sorting-associated Protein 35, hVPS35, Maternal-embryonic 3, Vesicle Protein Sorting 35, MEM3, TCCCTA00141)

Cat no: V0650-24C

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