XPG (Xeroderma Pigmentosum Type G, ERCC5, ERCM2, Excision Repair Cross Complementing Rodent Repair Deficiency, UVDR, Xeroderma Pigmentosum Complementation Group G, XPGC, DNA Repair Protein Complementing XP-G Cells, DNA Excision Repair Protein ERCC-5)

Patients with XPG, produce truncated proteins encoded by the ERCC5 gene severly lacking endonuclease activity. Patients with XPG are extremely photosensitive presenting with erythrema and blistering often giving rise to skin cancers. Skin fibroblasts are exquisitively sensitive to UV due to severe deficiency in nuceotide excision repair.\n\nApplications:\nSuitable for use in Immunohistochemistry (frozen), Immunoprecipitation, and Western Blot. Other applications not tested. \n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

X1048-01A

Size

100ug

Applications

IHC, IP, WB

Hosts

Mouse

Form

Supplied as a liquid in PBS. No preservative added.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a

References

1. Evans, E. et al. (1997) Open complex formation around a lesion during nucleotide excision repair provides a structure for cleavage by human XPG protein. EMBO J. 16:625-38. 2. O'Donovan, A. et al. (1994) Isolation of active recombinant XPG protein, a human DNA repair endonuclease. J Biol Chem. 269:15965-8. 3. Nouspikel, T. et al. (1997) A common mutational pattern in Cockayne syndrome patients from xeroderma pigmentosum group G: implications for a second XPG function. Proc Natl Acad Sci USA. 94:3116-21. 4. Caonstaintinou, A. et al. (1999) Conserved residues of human XPG protein important for nuclease activity and function in nucleotide excision repair. J Biol Chem.274:5637-48. 5. Araujo, S. J., Nigg, E. A. and Wood, R. D. (2001) Strong functional interactions of TFIIH with XPC and XPG in human DNA nucleotide excision repair, without a preassembled repairosome. Mol Cell Biol. 21:2281-91.

Additional Info

Recognizes the Xenoderma Pigmentation protein G.