Categories Molecular Biology
Under physiological conditions, most protein interactions are transient, posing significant challenges for their study. Crosslinking reagents resolve this by...
Co-immunoprecipitation (Co-IP) is a classic method based on the principle that antibodies specifically recognize particular antigens for studying...
Accurate mass determination can be applied to all areas encompassed by chemistry and Accurate mass determination can be applied to all areas involved in...
With the widespread application of flow cytometry (FC) and solid-phase technology, along with the revolutionary advancements in microsphere-based...
Peptide purity is defined as the ratio of the target peptide to all analytes, essentially reflecting the proportion of the target peptide to impurities. It...
The utilization of the bottom-up approach in proteomics is often constrained by the incomplete characterization of alternatively spliced isoforms, a wide...
The identification and analysis of single proteins or protein mixtures via mass spectrometry, as well as the analysis of individual protein sequences, are...
The amino acid sequence of proteins forms the basis for determining protein structure and function. Protein sequencing, which determines the types and...
Protein mutation refers to the alteration in the amino acid composition or sequence of a protein, which subsequently leads to changes in the structure and...
Protein purity refers to the relative proportion of the target protein in a protein sample, typically expressed as percentage. The higher the purity, the...
Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is frequently employed in protein expression analysis and other scientific research...
Molecular weight is a basic physicochemical parameter of organic compounds, generally referring to the relative molecular mass, which is the sum of the...
Shotgun proteomics, also known as shotgun method, refers to the utilization of bottom-up proteomics techniques to study all proteins present in complex...
Membrane proteins, such as receptors and ion channels, serve as crucial regulators of cellular function. Membrane proteins constitute two-thirds of known...
Proteins serve as the primary functional components of cells. Investigating proteins allows for a deeper understanding of biological processes, disease...
Proteins serve as the carriers of life substances and executors of biological functions, playing a crucial role in maintaining normal physiological...
Unknown protein identification refers to the process of identifying, studying, and annotating proteins that are not fully understood in terms of function and...
Interacting protein mass spectrometry identification is a method utilizing mass spectrometry technology to study protein-protein interactions. It separates...
Protein tandem mass spectrometry (MS/MS) is an efficient, precise, and sensitive method for qualitative and quantitative identification of proteins based on...
Gel electrophoresis is an important protein analysis method, which includes multiple techniques such as sodium dodecyl sulfate-polyacrylamide gel...
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