Acid Sensitive Ion Channel 1 (ASIC1, BNAC 2, Amiloride-sensitive brain sodium channel, ACCN2)

ASIC1 is a member of a family of Na +channels that are activated by external protons. The family includes another three members ASIC2, ASIC3 and ASIC4. The ASICs are in fact part of a larger superfamily termed epithelial Na +channels (EnaC) and share with it the same basic characteristics: two transmembrane spanning domains, a large extracellular domain and short intracellular N and C termini. There are two recognized splice variants of the ASIC1 gene that differ on their N-termini, ASIC1a and ASIC1b that have different tissue distributions and functions. The ASIC1 responds to a decrease in extracellular pH with an inward cation current that is quickly inactivated despite the continuous presence of protons in the medium. Lately, ASIC1 has been implicated in processes such as learning and memory in the central nervous system. \n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution: \nWestern Blot (Rat brain membranes): 1:200. \nImmunohistochemistry: Rat brain sections \nOptimal dilutions to be determined by the researcher.\n\nControl Antigen:\nA0570-05A\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0570-05

Size

50ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide before the addition of glycerol to 40%.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Kellenberger, S., et al., Physiol. Rev. 82: 735 (2002). 2. Wemmie, J. A. et al. (2002) Neuron 34, 463. 3. Wemmie, J. A. et al. (2003) J. Neurosci. 23, 5496.

Additional Info

Recognizes rat ASIC1a and ASIC1b. Species Sequence Homology: human: identical; mouse: 19/20 residues identical.