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Adipophilin (ADFP, Adipose Differentiation-related Protein)

Adipophilin (ADFP, Adipose Differentiation-related Protein)

Cat no: A0887-01A

Supplier: United States Biological
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Milk lipid globules from humans, cows and rats contain a protein identified as adipocyte differentiation-related protein (ADFP). It is associated with the globule surface membrane material. This protein, previously believed to be specific to adipocytes, is a major constituent of the globule surface and is present in a detergent-insoluble complex that contains butyrophilin and xanthine oxidase. ADFP (Adipophilin) is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells. In tissues, however, expression of adipophilin is restricted to specific cell types, such as lactating mammary epithelial cells, adrenal cortex cells, Sertoli and Leydig cells of the male reproductive system, and steatosis or fatty change hepatocytes in alcoholic liver cirrhosis. ADFP may be a possible new marker for the identification of specialized differentiated cells containing lipid droplets and for diseases associated with fat-accumulating cells. Applications: Suitable for use in Western Blot, Immunofluorescence, Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml Immunohistochemistry (Paraffin): 1:200. Staining was observed on the membrane of mouse liver cells. Antigen retrievak with sodium citrate buffer, pH 6.0 is recommended prior to staining paraffin tissues. Immunofluorescence (IC): 1:50-1:200 Membrane staining was observed in HeLa cells Optimal dilutions to be determined by the researcher. Positive Control: Mouse and human liver lysate Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: A0887-01A
Reactivities: Human, Mouse
Hosts: Rabbit
Applications: Immunofluorescence, Immunohistochemistry, Western Blot
Size: 100ul
Form: Supplied as a liquid in PBS, pH 7.4, 30% glycerol, 0.05% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
Alternative names: ADRP
References: 1. Son S-H, Goo Y-H, Chang BH, Paul A Perilipin 2 (PLIN2)-Deficiency Does Not Increase Cholesterol-Induced Toxicity in Macrophages PLoS ONE 2012 [PMID: 22427949] (WB, Mouse). 2. Wohlers LM, Jackson KC, Spangenburg EE. Lipolytic signaling in response to acute exercise is altered in female mice following ovariectomy. J Cell Biochem. 2011 Aug 3. [PMID: 21815195]
Additional info: Recognizes mouse Adipophilin at ~51kD. Species Crossreactivity: human. Species sequence homology: rat, 88%.

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