ADNP (Activity-dependent neuroprotective protein-1) is a member of a small family of VIP-induced neurotrophic factors. Although its predicted MW is 124kD, it runs anomalously at 150kD in SDS-PAGE. ADNP is known to be expressed by endothelium, astrocytes, neurons, macrophages, monocytes and B cells, and serves to down-regulate inflammation in immune cells, while acting to promote neuronal survival in response to stress or injury. Human ADNP is 1102aa in length. It has no definitive signal sequence, but is reported to be found both extracellularly and intracellularly, where it shuttles between cytoplasm and nucleus. Full-length ADNP contains nine consecutive C2H2-type zinc finger regions (aa74-686), followed by a DNA-binding homeobox domain (aa754-814). There are 12 utilized phosphoserine sites, two C-terminal acetylated lysines, an NLS (aa716-733), an NES (aa788-804) and one NAPVSIPQ (NAP) motif (aa354-361) that likely mediates the ADNP neuroprotective function. Over aa941-1102, human ADNP shares 93aa identity with mouse ADNP.
Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry: 5-15ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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