ADP Ribosylation Factor (ARF)

The ADP-ribosylation factor (Arf) family comprises a group of structurally and functionally conserved 21kD proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. Arf is involved in intracellular protein traffic to and within the Golgi complex. Arf has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D. The Arf family is divided functionally into the Arf and the Arf-like (Arl) proteins. Arfs share more than 60% sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily. \n\nApplications: \nSuitable for use in Immunohistochemistry, Western Blot, Immunoprecipitation.\n\nRecommended Dilution:\nImmunohistochemistry: 1:500\nWestern Blot: 1:500\nNote: Not suitable for neutralizing ARF in solution.\n\nCellular Localization: Golgi apparatus\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

A0902-10A

Size

100ul

Applications

IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Can

Form

Liquid. Ascites diluted in PBS containing 0.05% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

Intracellular distribution of Arf proteins in mammalian cells. Arf6 is uniquely localized to the plasma membrane: M.M. Cavenagh, et al.; J. Biol. Chem. 271, 21767 (1996)

Additional Info

Recognizes human, mouse, rat, dog and hamster ARF1, ARF3, ARF5 and ARF6. Detects a band of ~21kD by Western blot.