AMP-activated protein kinase (AMPK) is the downstream component of a protein kinase cascade that acts as an intracellular energy sensor maintaining the energy balance within the cell. This pivotal role of AMPK places it in an ideal position for regulating whole-body energy metabolism. AMPK is activated in response to ATP depletion, which causes a concomitant increase in the AMP:ATP ratio . Once activated, it phosphorylates several downstream substrates, the overall effect of which is to switch off ATP-consuming pathways (e.g. fatty acid synthesis and cholesterol synthesis) and to switch on ATP-generating pathways (e.g. fatty acid oxidation and glycolysis). The protein also plays a part in protecting the body from metabolic diseases such as type 2 diabetes and obesity. Structurally AMPK is a heterotrimeric complex comprising a catalytic subunit (alpha) and two regulatory subunits (beta and gamma).
Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry (Formalin fixed paraffin embedded): 1:50-1:100
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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