Adrenergic Receptor, beta 2 (Beta-2 Adrenergic Receptor, Beta-2 Adrenoreceptor, Beta-2 Adrenoceptor, ADRB2, ADRB2R, B2AR)

The b-adrenoceptors can be divided into b1, b2, b3 and b4-adrenoceptors defined in terms of agonist potencies, b2-adrenoceptors displayed a higher selectivity for nor-adrenaline than for adrenaline. B2-receptors are mainly postsynaptic and are located on a number of tissues including blood vessels, bronchi, GIT, skeletal muscle, liver and mast cell. Activation results in vasodilatation, bronchodilation, relaxation of the GIT, glycogenolysis in the liver, tremor in skeletal muscle and inhibition of histamine release from mast cells. Transduction is via G-proteins coupled to the intracellular second messenger adenylate cyclase. B-receptors are positively coupled to adenylate cyclase via activation of Gs G-protein, however activation of the b2-adrenoceptors results in stimulation and inhibition of adenylate cyclase. The b2-receptor selective agonists are widely used in the treatment of asthma and other related bronchospastic conditions. They are commonly used in the treatment of angina pectoris, cardiac arrhythmia and for the long-term treatment of patients who survive myocardial infarction. B-receptor antagonists have also been used as anti-hypertensive for a number of years. Beta-blockers have also proven useful in the treatment of conditions such as migraine, anxiety disorders, hyperthyroidism, alcohol withdrawal and when applied topically are useful in the treatment of glaucoma and ocular hypertension.\n\nApplications:\nSuitable for use in Flow Cytometry, ELISA and Functional Assays. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: A431 human epidermoid cells in suspension for 1h at 4 degrees C in PBS containing monoclonal antibody (260nM). After 1h of incubation, the cells were fixed with 2% formaldehyde\nFunctional Assays: functions as an agonist in neonatal rat cardiomyocytes. Furthermore, Fab fragments of agonist-like antibody 6H8 behave as antagonist\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: Human epidermoid carcinoma cell A431 line\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0906-89

Size

100ug

Applications

ELISA, FC

Hosts

Mouse

Reactivities

Hum, Rat, GP

Form

Supplied as a liquid in PBS, 0.1% BSA.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Lebesgue, D et al; An agonist-like monoclonal antibody against the human beta2-adrenoceptor. Eur J Pharmacol 1998, 348: 123. 2. Mijares, A et al; From agonist to antagonist: Fab fragments of an agonistic-like monoclonal anti- beta2-adrenoceptor antibody behaves as antagonists. Mol Pharmacol 2000, 58: 373.

Additional Info

Recognizes human Beta2-adrenoceptor. Species Crossreactivity: rat and guinea pig.