Adrenomedullin (ADM; also AM) is a secreted, monomeric, 6kD member of the Adrenomedullin family of molecules. It is widely expressed, being found in smooth muscle cells, endothelium, adrenal medulla chromaffin cells, fibroblasts and keratinocytes. ADM has multiple functions, including vasodilation, the maintenance of vascular integrity, and the suppression of inflammatory mediator secretion. The human ADM preproprecursor is 185aa in length. It contains a 21aa signal sequence, a processed 20aa peptide termed PAMP (aa22-41), an N-terminal propeptide (aa45-92), the ADM precursor (amidation is required for maturation) (aa 95-147), and a C-terminal propeptide (aa148-185). The ADM precursor with a terminal Gly147 circulates naturally with bioactive, mature amidated ADM (aa95-146). Depending upon the tissue, truncated forms of ADM likely also occur, including variants spanning (aa120-146) and 128-146. Over (aa22-147), the human ADM proprecursor shares 70% aa identity with mouse ADM proprecursor.
Applications:
Suitable for use in Direct ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilutions:
Immunohistochemistry: 8/25ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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