Agmatine (Guanidinium Analogue)

Applications: \nSuitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications have not been tested.\n\nRecommended Dilution:\nImmunohistochemistry (paraffin): 1:100 for 0.1-2.5% glutaraldehyde fixed tissues. Optimal fixation: 0.1-2.5% glutaraldehyde, 1% formalin. Minimum glutaraldehyde: 0.1%; works on paraffin embedded tissue (fixed with glutaraldehyde) but preferably epoxy embedded specifically developed for post-embedding protocols. Living, isolated goldfish retinas were maintained for 10 minutes in an oxygenated physiological solution that preserves normal neuronal activity under constant lighting. The medium also contained 10mM agmatine. After the incubation period retinas were fixed in a conventional 2.5% glutaraldehyde buffer and processed for immunohistochemistry using 250nm sections probed with a gold-labeled secondary antibody. The treated retinas were exposed to 125M kainate during the incubation. Kainate opens both AMPA and kainate selective ionotropic channels through which agmatine can enter cells.\nImmunocytochemistry: On cells with glutamate-gated ion channels. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. \n

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SPECIFICATIONS

Catalog Number

A1059-56

Size

2000Tests

Applications

ICC, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 1% goat serum, 0.05% thimerosal.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Hille, B., Ionic channels of excitable membranes. Sinauer Assoc., Sunderland, MA (1984). 2. Li, et al., Science 263: (1994) 3. Quik, M., Brain Research 325: 79

Additional Info

Recognizes Agmatine. Does not crossreact with arginine, glutamate or other amino acids. Is expected to recognize agmatine in mose vertebrate species.

Alternative Names

(4-aminobutyl)guanidine \n