Home  >  Products  >  Aurora 1, Recombinant Human (STK12, Serine Threonine Kinase 12, Aurora B, AIK2, AIM1, AurB, IPL1, ARK2, Aurora Related Kinase 2)

Aurora 1, Recombinant Human (STK12, Serine Threonine Kinase 12, Aurora B, AIK2, AIM1, AurB, IPL1, ARK2, Aurora Related Kinase 2)

Cat no: A4189-26A


Supplier: United States Biological
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Aurora Kinase B is a member of a family of mitotic serine/threonine kinases. Aurora Kinase B associates with chromosomes during prophase prior to relocalizing to the spindle at anaphase. Aurora Kinase B regulates chromosome segregation through the control of microtubule-kinetochore attachment and cytokinesis. Expression of Aurora Kinase B during the G2/M phase transition is tightly coordinated with histone H3 phosphorylation, while overexpression in many kinds of cancers. Recombinant human Aurora Kinase B, fused to His-tag at N-terminus, , expressed in E. coli and purified by using conventional chromatography techniques. Source: Recombinant human Aurora Kinase B, aa1- 344, His-tagged, expressed in E. coli AA Sequence: MGSSHHHHHH SSGLVPRGSH MAQKENSYPW PYGRQTAPSG LSTLPQRVLR KEPVTPSALV LMSRSNVQPT AAPGQKVMEN SSGTPDILTR HFTIDDFEIG RPLGKGKFGN VYLAREKKSH FIVALKVLFK SQIEKEGVEH QLRREIEIQA HLHHPNILRL YNYFYDRRRI YLILEYAPRG ELYKELQKSC TFDEQRTATI MEELADALMY CHGKKVIHRD IKPENLLLGL KGELKIADFG WSVHAPSLRR KTMCGTLDYL PPEMIEGRMH NEKVDLWCIG VLCYELLVGN PPFESASHNE TYRRIVKVDL KFPASVPMGA QDLISKLLRH NPSERLPLAQ VSAHPWVRAN SRRVLPPSAL QSVA Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A4189-26A
Size: 100ug
Form: Supplied as liquid in 20mM Tris-HCl buffer, pH8.0, 0.5mM DTT, 20% glycerol, 0.1mM EDTA, 0.1mM EGTA, 0.1M sodium chloride, 0.1mM PMSF
Purity: ~90% by SDS-PAGE Purified by immunoaffinity chromatography.

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