Avidin D, Rhodamine600 (XRITC) labeled, is made from ultrapure Avidin D and XRITC (a substituted form of rhodamine isothiocyanate). Rhodamine600 Avidin D also produces a red fluorescence but has absorption and emission maxima of about 25nm longer wavelength than the TMRITC-labeled Avidin D. Rhodamine600(XRITC) Avidin D has an absorption maximum at about 575nm and a maximum emission at about 600nm. This feature can provide an advantage in double label techniques when using fluorescein as a second label since there is less overlap in spectra with rhodamine600 and consequently, less opportunity for energy transfer effects. Rhodamine600 Avidin D is also the reagent of choice when using krypton laser cell sorting. Rhodamine600 has about the same quantum yield and photobleaching characteristics as the tetramethylrhodamine label.
Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry: 5-30ug/ml
Optimal dilutions to be determined by the researcher.
Excitation:
583-590nm
Emission:
595-601nm
Moles of Rhodamine/Avidin D:
2.5
Storage and Stability:
May be stored at 4 degrees C. For long-term storage, aliquot and store at 4 degrees C. Do not freeze. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
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