Bcl 2 (B Cell Leukemia 2, B Cell Leukemia/lymphoma 2, B Cell Lymphoma 2, B-cell CLL/lymphoma 2, Bcl2, Bcl-2 Protein, Apoptosis Regulator Bcl 2, Apoptosis Regulator Bcl2)

B0807-03M is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. This antibody may also be used in distinguishing between those follicular lymphomas that express Bcl 2 protein and the small number in which the neoplastic cells are Bcl 2 negative. Anti-Bcl 2 has been used as a predictive biomarker for recurrence in the case of radical prostatectomy for prostate cancer, and has shown promise as a prognostic marker in the case of cancer of the breast and non-small cell carcinoma of the lung.\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (Paraffin, frozen): 1:5-1:25. Cytoplasmic staining pattern.\nOptimal dilutions to be determined by the researcher. \n\nImmunohistochemistry Preparation and Pretreatment:\n1. Cut 3-4um sections of formalin-fixed paraffin embedded tissue and place on positively charged slides; dry overnight at 58C.\n2. Deparaffinize, rehydrate and epitope retrieve; the preferred method is the use of Heat Induced Epitope Retrieval (HIER) techniques in conjugation with a pressure cooker. The preferred method allows for stimultaneous deparaffinization, rehydration and epitope retrieval. Upon completion, rinse with 5 changes of ddH2O.\n3. If using a HRP detection system, place slides in peroxide block for 10 minutes; rinse. \n\nPositive Control: \nLymph Node, Tonsil\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

B0807-03M

Size

1ml

Applications

ELISA, IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, protein base, sodium azide.

P Type

Mab

Purity

Supernatant

Isotype

IgG1,k

References

1. Tsujimoto, Y, et al., Prac Natl Acad Scie (USA) 1986;83:5214-5218. 2. Cleary , ML, et al., Cell 1986;47:19-28 3. Pezzella, F, et al., Am J Pathol 1990;137:225-232. 4. Hockenbery, D, et al., Nature 1990;348:334-336. 5. Moul JW et al. Eur urol. 1999;35(5-6): 399-407. 6. Ciocca DR, Elledge R. Endocrine 2000 Aug;13(1): 1-10. 7. Martin B et al. Br J Cancer. 2003 Jul 7;89(1): 55-64.

Additional Info

Recognizes human Bcl 2. Shows consistent negative reactions on reactive germinal centers and positive staining of neoplastic follicles in follicular lymphoma.