BD1, Mouse (beta Defensin-1)

Mouse and rat beta-defensin-1 is synthesized from 69aa precursor (mature peptide 33-69aa). The sequence of mature MBD-1 is: DQY KCL QHG GFC LRS SCP SNT KLQ GTC KPD KPN CCK S, 37aa. Was synthesized and disulfides were formed in their natural orientation). \n\nAntimicrobial peptides are a common mechanism of host defense utilized by a variety of species, from insects to humans. Defensins are a large family of broad-spectrum antimicrobial peptides, identified originally in leukocytes of rabbits and humans. Defensins, cationic/polar peptides (30-35 aa; 3-4kD), are distinguished by a conserved tri-disulfides and a largely b-Sheet structure. Defensins, expressed at the cell surface, have been hypothesized to function as a biochemical barrier against microbial infection by inhibiting colonization of the epithelium by wide range of pathogenic microorganisms. In leukocytes, these peptides are stored in cytoplasmic granules and are released into phagolysosomes where they contribute to the killing of engulfed microorganisms.\n\nThe genes encoding human a and b -defensins are clustered in a contiguous segment of chromosome 8p23. Defensins are classified into two families designated a-and b-based on distinctive, although similar, tri-disulfide linkages in the peptides. b-defensins are slightly larger and differ in the position and arrangement of 3 disulfides. In humans, six a -defensin (cryptidins), HD 1-6 (HD1-4 are also known as HNP1-4 for Human Neutrophil Peptides), and two b -defensins, HBD-1 and HBD-2, have been identified to date. Rat (RBD-1 and RBD-2) and mouse (MBD1-4) homologues of the human beta-defensin have also been identified.a-defensins are encoded by genes designated DEFA1-6, whereas human b-defensins are encoded by the DEFB1 and DEFb2 genes.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

B0899-01

Size

50ug

Applications

ELISA

Form

Supplied as a liquid in PBS, pH 7.2.

Purity

Purified (same/more than)95% by HPLC

References

1. Huttner Km et al (1997) FEBS Lett. 413, 45-69; 2. Liu L et al (1997) Genomics 43, 316-320; 3. Bartels J et al (1997) Nature 387, 861; 4. Ganz T (1999) Science 286, 420; Yang D et al (1999) Science 286, 525.