Bmp10, Recombinant, Mouse, CF (Bone Morphogenetic Protein 10, BMP-10)

Bone morphogenetic protein 10 (BMP-10), along with BMP-9, GDF-5, -6, and -7, belongs to a subgroup of TGF-b superfamily proteins that signal through heterodimeric complexes composed of type I and type II BMP receptors. Proteolytic removal of the propeptide from the 60kD proprotein yields a 12kD mature BMP-10 which forms disulfide-linked nonglycosylated homodimers. In transfectants, BMP-10 can also be secreted as a proprotein which binds to matrix fibrillin. Mature mouse BMP-10 shares 98% and 100sequence identity with human and rat BMP-10, respectively, and 48%-64% with mouse BMP-9, GDF-5, -6, and -7. BMP-10 is critical for the proper development of the heart and first appears at the onset of trabeculation and chamber formation. Homozygous BMP-10 knockout mice die in utero due to arrested cardiac development. BMP-10 is required for maintaining expression of the cardiogenic transcription factors NKX2.5 and MEF2C in developing myocardium and promoting the Notch-dependent growth of embryonic cardiomyocytes. NKX2.5 itself negatively regulates BMP-10 expression in cardiac myocytes. BMP-10 in the postnatal heart promotes increased cardiomyocyte and heart size and is upregulated in hypertrophied ventricles. Mature BMP-10 accumulates within cardiomyocytes in association with the sarcomere protein Tcap. BMP-10 induces signaling through ALK-1, BMPR-IA, BMPR-IB, and BMPR-II in transfectants and non-cardiac cell lines. Deletion of BMPR-IA or BMP-10 causes similar cardiac morphogenetic abnormalities. In dermal endothelial cells, BMP-10 induces migration, proliferation, and gene expression typically associated with ALK-1.\n\nSource: \nChinese Hamster Ovary cell line, CHO-derived Asn314-Arg421.\n\nSDS-PAGE:\n10kD, reducing conditions\n\nActivity:\nMeasured by its ability to induce alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells. The ED50 for this effect is typically 15-75ng/ml.\n\nEndotoxin Level: (same/less than)1EU/1ug (LAL)\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

B2553-37H

Size

25ug

Reactivities

Mouse

Form

Supplied as a lyophilized powder in HCl. Reconstitute with 4mM HCl.

Purity

~95% (SDS-PAGE, reducing conditions)

References

1. Chen, D. et al. (2004) Growth Factors 22:233. 2. Miyazono, K. et al. (2005) Cytokine Growth Factor Rev. 16:251. 3. Schneider, M.D. et al. (2003) Cytokine Growth Factor Rev. 14:1. 4. Mazerbourg, S. et al. (2005) J. Biol. Chem. 280:32122. 5.David, L. et al. (2007) Blood 109:1953. 6. Sengle, G. et al. (2008) J. Biol. Chem. 283:13874. 7. Neuhaus, H. et al. (1999) Mech. Dev. 80:181. 8. Chen, H. et al. (2004) Development 131:2219. 9.Chen, H. et al. (2006) J. Biol. Chem. 281:27481. 10. Srivastava, D. and Olson, E.N. (2000) Nature 407:221. 11. Pashmforoush, M. et al. (2004) Cell 117:373. 12. Grego