Bone Morphogenetic Protein 10, Recombinant, Human (BMP10)

The mature recombinant human BMP-10, generated by the proteolytic removal of the signal peptide and the propeptide, is a disulfide-linked homodimeric protein. Based on N-terminal sequencing, the mature recombinant human BMP-10 starts at Asn 317. The monomer has a calculated molecular mass of 12.2kD and migrates as an approximately 12.2kD protein in SDS-PAGE under reducing conditions. \n\nSource: \nRecombinant corresponding to Asn317-Arg424 from human BMP10 expressed in CHO cells (O95393).\n\nActivity: \nMeasured by its ability to induce alkaline phosphatase synthesis by MC3T3E1 osteoblastic cells (Mazerbourg, S. et al., 2005, J. Biol. Chem. 280:32122). The ED50 for this effect is typically 15-60ng/mL. \n\nReconstitution: \nIt is recommended that sterile 4mM HCI containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 10ug/mL. \n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile 4mM HCI, with (same/more than)0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

B2553-37A

Size

25ug

Form

Supplied as a lyophilized powder from 30% Acetonitrile, 0.1% TFA, 1.25mg BSA. Reconstitute to 10ug/ml in sterile 4mM HCI, with (same/more than)0.1% HSA or BSA.

Purity

(same/more than) 97%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: (same/less than)1.0 EU/ug o(LAL).