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Bone Morphogenetic Protein 4, 293-408aa, Recombinant, Human (BMP4, DVR4)

Cat no: B2553-21A

Bone Morphogenetic Protein 4, 293-408aa, Recombinant, Human (BMP4, DVR4)

Bone morphogenetic protein 4 (BMP-4) is a member of the bone morphogenetic protein family which belongs to the TGF-b superfamily. This protein is a vital regulatory molecule that functions throughout bone and cartilage development, specifically tooth development, limb formation, bone induction, and fracture repair. In human embryonic development, BMP- 4 is required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. And it is overexpressed in patients with fibrodysplasia ossificans progressiva. Recombinant BMP-4 was expressed as insoluble protein aggregate in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. \n\nEndotoxin Level: \n(same/less than) 1.0 EU per 1 ug of protein (determined by LAL method)15% SDS-PAGE (3ug)\n\nMolecular Weight: 13.2kD (117 aa)\n\nAA Sequences:\nMSPKHHSQRA RKKNKNCRRH SLYVDFSDVG WNDWIVAPPG YQAFYCHGDC PFPLADHLNS TNHAIVQTLV NSVNSSIPKA CCVPTELSAI \nSMLYLDEYDK VVLKNYQEMV VEGCGCR\n\nStorage and Stability:\nFor long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

B2553-21A

Size

100ug

Form

Supplied as a liquid in 10mM sodium citrate buffer, pH 3.5, 10% glycerol.

Purity

(same/more than) 95% by SDS-PAGE

References

1. Moon BS., et al. (2009) Exp Mol Med. 41(2):116-25. 2. Li BC., et al. (2009) J Trauma. 66(2):450-6

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Applications

ELISA

Reactivities

Hum

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Applications

IF

Hosts

Mouse

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Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

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Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

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Applications

ELISA, FC, IHC, WB

Hosts

Mouse

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Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

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Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

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