Bone Morphogenetic Protein 9 (BMP9, Growth and Differentiation Factor 2, GDF2)

Bone Morphgenetic Protein 9 (BMP-9), also known as GDF-2, is a member of the BMP subgroup of the TGF-beta superfamily proteins. BMP-9 regulates the development and function of a variety of embryonal and adult tissues. It also promotes hepatic lipid metabolism and inhibits glucose production. Unlike with other BMP family proteins, the propeptide remains associated with the mature peptide after proteolytic cleavage and does not interfere with BMP-9 biological activity. Mature human and mouse BMP-9 share 96% aa sequence identity.\n \nApplications:\nSuitable for use in Western Blot, ELISA and Neutralization. Other applications not tested.\n\nRecommended Dilution: \nWestern Blot: 1ug/ml using recombinant human BMP-9 under non-reducing conditions only \nELISA: 2-8ug/ml (Capture); 0.1-0.4ug/ml (Detection)\nNeutralization of human BMP-9 bioactivity: Measured by its ability to neutralize BMP-9-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The Neutralization Dose (ND50) is typically 0.01-0.04ug/ml in the presence of 2ng/ml Recombinant Human BMP-9.\nOptimal dilution determined by the researcher. \n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

B2553-36

Size

100ug

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.

P Type

Mab

Purity

Purified by Protein G affinity chromatography. \nEndotoxin: < 0.1EU/ug (LAL).

Isotype

IgG2b

Additional Info

Recognizes human Bone Morphogenetic Protein 9. Does not cross react with recombinant human BMP-1, -1.1, -2, -3, -3b, -4, -5, -6, -7, -8 or -10. In direct ELISAs, ~80% cross-reactivity with recombinant mouse BMP-9 and 10% cross-reactivity with recombinant mouse BMP-10 is observed.