BRAF (Serine/Threonine-protein Kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf Murine Sarcoma Viral Oncogene Homolog B1, BRAF1, RAFB1)

B-Raf, also know as c-Rmil, is a Serine/Threonine protein kinase member of the Raf family which includes Raf-1 and A-Raf. B-Raf is involved in the transduction of mitogenic signals from the cell membrane to the nucleus (1). Composed of three conserved region (CR1, CR2, CR3) B-Raf is expressed primarily in the brain and in the nervous system (2). It has been observed that MAPK is activated by B-Raf in response to nerve growth factor (3). More than 60% of malignant melanomas were found to contain a specific mutation, B-Raf (V599E) the product of which possesses constitutive kinase activity (4). Mutations in B-Raf have also been identified in lung cancer and non-Hodgkin lymphoma.\n\nApplications: \nSuitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:50\nWestern Blot: 1:1,000-5,000\nImmunohistochemistry (paraffin): 1:250-500\nImmunoprecipitation: 1:50\n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

B2680-03B

Size

100ul

Applications

FC, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Hagemann C., Rapp U. R. Cell Res., 253: 34-46, 1999 2.Heidecker et al 1990 Mol. Cell. Biol. 10:2503-2512. 3. Jaiswal, R. K., Moodie, S. A., Wolfman, A., and Landreth, G. E. (1994) Mol. Cell. Biol. 14, 6944-6953 4. Brose et al. Cancer Res., 62: 6997-7000, 2002

Additional Info

Recognizes human B-Raf. Species crossreactivity: mouse. Recognizes rat B-raf by Western Blot.