Brain-Derived Neurotrophic Factor, Recombinant, Human (BDNF, Abrineurin, MGC34632)

Source:\nRecombinant corresponding to 2x119aa of human Brain-derived Neurotrophic Factor expressed in E.coli is a homodimer, non-glycosylated, polypeptide chain.\n\nMolecular Weight:\n~27kD\n\nBiological Activity:\nThe ED50, calculated by the dose-dependent induction of ACHE (acetylcholine esterase)in rat basal forebrain primary septal culture is 50nug/ml\n\nDimers and Aggregates:\n(same/less than)1% (SDS-PAGE)\n\nProtein Content:\nProtein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280nm using the absorbency value of 1.6 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of BDNF as a Reference Standard.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

B2700-01K

Size

2ug

Form

Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to (same/more than)0.1mg/ml.

Purity

~96% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: (same/less than)0.1ng/ug (IEU/ug)

References

1. NMDA receptor activation modulates programmed cell death during early post-natal retinal development: a BDNF-dependent mechanism. J Neurochem 2005 Oct;95(1):244-53. 2. Cellular levels of TrkB and MAPK in the neuroprotective role of BDNF for embryonic rat cortical neurons against hypoxia in vitro. Int J Dev Neurosci 2005 Oct;23(6):515-21. 3. Increased expression of BDNF and proliferation of dentate granule cells after bacterial meningitis. J Neuropathol Exp Neurol 2005 Sep;64(9):806-15. 4. BDNF acutely modulates synaptic transmission and calcium signalling in developing cortical neurons. Cell Physiol Biochem 2005;16(1-3):69-76. 5. Area-specific effects of brain-derived neurotrophic factor (BDNF) genetic ablation on various neuro nal subtypes of the mouse brain. Brain Res Dev Brain Res 2005 May 12;156(2):111-26. 6. The change of the neuron-glia differentiation rate in human neural precursor cells (HPCs) and Ad-BDNF-/-GDNF-infected HPCs following the administration of a neurotoxin. Neurosci Lett 2005 Oct 21;387(2):100-4.