Brain-Derived Neurotrophic Factor, Recombinant, Human (BDNF)

Human Brain-Derived Neurotrophic Factor is a homodimer, non-glycosylated, polypeptide chain containing 2 x 119 amino acids. The sequence of the first five N-terminal amino acids was determined to be Met-His-Ser-Asp-Pro. Contains less than 1% dimers and aggregates.\n\nSource: \nRecombinant Human Brain-Derived Neurotrophic Factor expressed in E. coli. \n\nMolecular Weight: 26,984\n\nActivity:\nThe ED50 as determined by the dose dependent induction of ACHE (acetylcholine esterase) in rat basal forebrain primary septal culture is 50ng/ml. \n\nEndotoxin: (same/less than)0.1 ng/ug\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Reconstitute with ddH2O. Aliquot and store at -20 degrees C. For long-term storage, add a carrier protein (0.1% HSA or BSA), Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

B2700-01H

Size

10ug

Applications

ELISA, WB

Form

Supplied as a lyophilized powder from 20mM sodium citrate buffer, pH 5.0. No preservative added. Reconstitute using sterile deionized water to a concentration (same/more than)100ug/ml.

Purity

~96% (HPLC, FPLC, reducing and non-reducing SDS-PAGE)