Casein Kinase 1 epsilon (Casein Kinase I epsilon, Casein Kinase I Isoform epsilon, CKI epsilon, CKIe, CK1 epsilon, CSNK1E, HCKIE, KC1E, Kinase CK1 epsilon, MGC10398)

The protein encoded by this gene is a serine/threonine protein kinase and a member of the casein kinase I protein family, whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins, including itself. This protein has been shown to phosphorylate period, a circadian rhythm protein. Two transcript variants encoding the same protein have been found for this gene. \n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 1:32,000\nWestern Blot: 0.3-1ug/ml\nOptimal dilutions to be determined by the researcher\n\nPositive Control:\nLysates of cell line HEK293\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 monthsat -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nPeptide Blocking:\nCorresponding peptide is available for Peptide Blocking studies. See C2085-54G-P. Antibody

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SPECIFICATIONS

Catalog Number

C2085-54G

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

References

Hammerlein A, Weiske J, Huber O. A second protein kinase CK1-mediated step negatively regulates Wnt signalling by disrupting the lymphocyte enhancer factor-1/beta-catenin complex. Cell Mol Life Sci. 2005 Mar;62(5):606-18.

Additional Info

Recognizes human CSNK1E at ~45kD. Species sequence homology: mouse, rat, canine.