Caspase 2, NT (ICH-1, Nedd2, Caspase-2, CASP-2, Neural Precursor Cell Expressed Developmentally Down-regulated Protein 2, NEDD-2, Protease ICH-1, CASP2)

Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1b and IL-18. Although phylogenetically similar to this subfamily, Caspase-2 is thought to be involved in stress-induced apoptosis. Caspase-2 has two major isoforms; overexpression on the long form results in apoptosis while that of the short form suppresses cell death.\n\nApplications:\nSuitable for use in Immunofluorescence, Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 2ug/ml\nWestern Blot: 1ug/ml\nImmunocytochemistry: 2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nRamos cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C2086-72R

Size

50ug

Applications

ICC, IF, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004;117:561-74. 2. Zhivotovsky B and Orrenius S. Caspase-2 funtion in response to DNA damage. Biochim. Biophys. Res. Comm. 2005;331:859-67. 3. Kuida K, Lippke JA, Ku G, et al. Altered cytokine export and apoptosis in mice deficient in interleukin-1 beta converting enzyme. Science 1995;267:2000-3. 4. Gracie JA, Robertson SE, and McInnes IB. Interleukin-18. J. Leukoc. Biol. 2003;73:213-224. 5. Lassus P, Opitz-Araya X, and Lazebnik Y. Requirement for caspase-2 in stress-induced apoptosis before mitochondrial permeabilization. Science 2002;10:1352-4. 6. Wang L, Miura M, Bergeron L, et al. Ich-1, and Ice/ced-3-related gene, encodes both positive and negative regulators of programmed cell death. Cell 1994;739-750.

Additional Info

Recognizes human Caspase-2 (NT). Species Crossreactivity: mouse.