Caspase 9 (ICE-LAP6, Mch6, Apaf 3)

Caspase 9 is a member of the Ced3/ICE cysteine protease caspase family that has a predicted molecular mass of 46kD and was originally isolated in human Jurkat T cells. Caspase 9 is critical for cytochrome c-dependent apoptosis and normal brain development. The active form of caspase 9 is composed of a 32-35kD subunit and a 10kD subunit that are proteolytically derived from procaspase 9 by caspase 3 (CPP3) and granzyme B. Procaspase 9 activation involves formation of a complex with the cofactor Apaf-1, cytochrome C, and deoxyadenosine triphosphate. Once activated, caspase 9 initiates a caspase cascade involving caspase 3,-6, and-7. Caspase 9 is expressed in a variety of tissues. The small heat shock protein Hsp27, has been shown to inhibit etoposide-induced apoptosis by preventing cytochrome C and dATP-triggered activity of caspase-9. \n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:2000\nImmunoprecipitation: 1:150\nImmunohistochemistry (Paraffin): 1:5000\nImmunocytochemistry: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C2088-01M

Size

50ul

Applications

ICC, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Can

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Release of caspase-9 from mitochondria during neuronal apoptosis and cerebral ischemia: S. Krajewski, et al.; PNAS 96, 5752 (1999). 2. Nuclear localization of procaspase-9 and processing by a caspase-3-like activity in mammary epithelial cells: P.M. Ritter, et al.; Eur. J. Cell Biol. 79, 358 (2000). 3. Temporal and spatial profile of Bid cleavage after experimental traumatic brain injury: G. Franz, et al.; J. Cereb. Blood Flow Metab. 22, 951 (2002).

Additional Info

Recognizes human Caspase 9. Detects both Procaspase 9 and the 35kD large subunit of activated Caspase 9. Recognizes the endogenous protein by Immunohistochemistry and Western Blot. Species Crossreactivity: canine, mouse and rat