CD1d, known as CD1.1 and Ly-38, is a 48kD type I membrane glycoprotein with structural similarities to MHC class I and is non-covalently associated with b2-microglobulin. In humans, CD1 family consists of group I proteins (CD1a, CD1b, and CD1c), group II (CD1d), and group III (CD1e). But CD1d is the only CD1 molecule has been found in mouse. Mouse CD1d has broad tissue distribution, and is found on leukocytes, dendritic cells, epithelial cells, and thymocytes. CD1d plays a role in non-peptide glycolipid antigen presentation to CD1d-restricted T cells. It has been shown that PKCd is a critical regulator of CD1d-mediated antigen presentation.
Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended Dilution:
Flow Cytometry: (same/less than)0.25ug/10e6 cells in 100ul volume
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.