CD1d is a glycoprotein expressed on cortical thymocytes, certain T-cell leukemias and various other antigen presenting cells. CD1d binds self and non-self glycolipids and presents them to T-cell receptors on natural killer cells. When activated, natural killer cells produce Th1 and Th2 cytokines.
Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 1:10-1:20; 10ul labels 10e6 cells in 100ul.
Immunohistochemistry: frozen sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C. For long-term storage, aliquot and store at 4 degrees C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.