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CD2 (E Rosette Receptor, T11, Lymphocyte Function Antigen 2, LFA-2, Sheep Erythrocyte Receptor)

Cat no: C2253-07J

CD2 (E Rosette Receptor, T11, Lymphocyte Function Antigen 2, LFA-2, Sheep Erythrocyte Receptor)

CD2 which is also known as E-rosette receptor, T11 and lymphocyte-function antigen-2 (LFA-2) is expressed on T cells, thymocytes, and subset of natural killer cells. Human CD2 functions as the receptor for sheep erythrocytes, human CD58 (LFA-3), and CD15s (Sialyl Lewis X). p56lck, p59fyn, CD3eta and CD3epsilon are tyrosine-phosphorylated\nafter CD2 stimulation. CD2 plays a role in T cell activation, T- or NK-cell-mediated cytolysis,\napoptosis in activated peripheral T cells, and regulation of T cell anergy. Normal B lymphocytes, monocytes or granulocytes do not express surface CD2 antigen, neither do common ALL cells. The antigen has been characterized as the receptor for sheep erythrocytes and CD2 monoclonal antibodies that inhibit E rosette formation. \n\nAntigen distribution: T cells (E+) 100% Thymocytes 95 + 3% Peripheral blood lymphocytes 82 + 5% NK cells (CD16+) 50 + 10% Granulocytes < 1% Monocytes < 1% B Cells (E-) < 1% \n\nFusion Partners: Spleen cells from immunized LOU rat were fused with cells of the NS1 myeloma cell line.\n\nApplications:\nFlow Cytometry: Neat\nELISA\nImmunoprecipitation \nOptimal working dilutions to be determined by researcher.\n\nFlow Cytometry: Use 50ul of the suggested working dilution to label 2x10e6 mouse lymphocytes in 100ul.

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SPECIFICATIONS

Catalog Number

C2253-07J

Size

2ml

Applications

ELISA, FC, IP

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a liquid in 0.2M Tris HCl pH 7.4, 5-10% fetal calf serum, 0.09% sodium azide.

P Type

Mab

Purity

Tissue Culture Supernatant.

Isotype

IgG2a

Additional Info

Recognizes the mouse CD2 antigen, expressed by T lymphocytes.

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

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