CD235a (Glycophorin A, GpA) (PE)

CD235a (Glycophorin A (GpA)) antigen is exclusively expressed on human erythroid cells and their progenitors. These antibodies may be effectively used for differentiation in acute leukemias e.g. presence of the CD235a antigen on leukemia cells indicates an early erythroid cell lineage of the tumor cells.\nCD235a antibodies are used to identify erythroid cells during hematopoietic differentiation. CD235a is not expressed on lymphoid or granulocytic progenitor cells and therefore is very useful as a marker for detection of the erythroid cell lineage [1,2]. CD235a seems to be absent from erythroid colony forming cells in bone marrow, but is present during maturation from pro-erythroid blast cells to mature erythrocytes. CD235a is clinically important in the classification of leukemias. Using Anti-CD235a antibodies, a distinction can be made between erythroleukemia and other (acute) leukemias like e.g. myeloid, lymphoid or undifferentiated leukemias [3,4]. Anti-CD235a is often used in combination with detection of H-antigen and/or anti-CD36 antibodies for additional characterization of early erythroid cells. Additional immunophenotyping of early erythroleukemias can be performed by detecting myeloid-associated antigens such as CD13, CD14, CD15, CD33 and CD34, to discriminate from lymphoid lineage-associated antigens like CD2, CD7, CD10 and CD19 [6].\nGpA is the carrier of blood group M and N specificities, which provides the cells with a large mucin-like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation. Another function for CD235a that has been proposed is as a membrane inhibitor of reactive lysis. CD235a Mab directed to papain-sensitive epitopes located in the extracelluar specific domain of GpA, agglutinating papain-treated cells. \n\nApplications:\nSuitable for use in Flow Cytometry. Other applications have not been tested.\n\nRecommended Dilution:\nFlow Cytometry: Neat-1:10. Use 10ul to label 10e6 cells in 100ul. Optimal working dilutions to be determined by researcher.\n\nHybridoma: Spleen cells from immunized DA rats were fused with cells of the Y3/Ag.1.2.3.\n\nRecommended Negative Controls: Rat IgG2b Negative Control: PE\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2548-98E

Size

100Tests

Applications

FC

Hosts

Rat

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, pH 7.2, 1% BSA, 0.1% sodium azide. Conjugated to R. Phycoerythrin (RPE). Reconstitute with 1ml sterile 40-50% glycerol, ddH2O.

P Type

Mab

Purity

Purified IgG

Isotype

IgG2b

References

1. Outram, S. et al . (1988). Erythromyeloid lineage fidelity is conserved in erythroleukaemia. Leuk. Res. 12: 651-7. 2. Jokiranta, T.S., Meri, S. (1993). Biotinylation of monoclonal antibodies prevents their ability to activate the classical pathway of complement. J. Immunol. 15: 2124-31. \nGeneral references:\n1. Andersson, L.C. et al. 1979. Glycophorin A as a cell surface marker of early erythroid differentiation in acute leukemia. Int.J.Cancer, 23, 717

Additional Info

Recognizes glycophorin A (CD235a), a major sialoglycoprotein of the human erythrocyte membrane.