Reacts with a common epitope of glycophorin A (CD235a) and glycophorin B (CD235b). Glycophorin A is the major sialoglycoprotein expressed on red blood cell membrane, and erythroid precursors. Glycophorin A shares strong homology with glycophorin B. This antibody recognizes human RBCs and erythroid precursors and is useful in erythroid cell development studies. Mature, non-nucleated red blood cells are characteristically glycophorin A positive, but CD45 and CD71 negative.
Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested
Recommended Dilution:
ELISA: 1:4000
Western Blot: 1-3ug/ml
Immunohistochemistry (Formalin-fixed, paraffin-embedded): 2ug/ml
Optimal dilutions to be determined by the researcher
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.