CD3 (CD3d, CD3e, CD3g, CD3z, Early T Cell Marker, T Cell Receptor Complex, TCR, T-cell Surface Glycoprotein CD3) (FITC)

T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits.\n\nCD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.\n\nApplications: \nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 20ul labels human blood cells in 100ul whole blood.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C2254-56D1

Size

100Tests

Applications

FC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH7.4, BSA, 15mM sodium azide. Labeled with fluorescein isothiocyanate (FITC).

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a

References

1. Leucocyte Typing III. McMichael A. J. et al (Eds.), Oxford University Press (1987). 2. Horejsi V. et al., Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74). Folia Biol. (Praha) 34, 23 (1988). PMID: 2968928\n3. Hilgert I. et al., Therapeutic in vivo use of the A1-CD3 monoclonal antibody. Transplantation 55, 435 (1993).PMID: 8434399\n

Additional Info

Recognizes the epsilon and delta chain of human CD3.

Alternative Names

CD247, T-cell Surface Glycoprotein CD3 delta Chain, T-cell Receptor T3 delta Chain, T3D, T-cell Surface Glycoprotein CD3 gamma Chain, T-cell Receptor T3 gamma Chain, T3G, T-cell Surface Glycoprotein CD3 epsilon Chain, T-cell Surface Antigen T3/Leu-4 epsilon Chain, T3E, T-cell Surface Glycoprotein CD3 zeta Chain, T-cell Receptor T3 zeta Chain, T3Z, TCRZ