CD33 (CD33 Molecule, gp67, Myeloid Cell Surface Antigen CD33, p67, Sialic Acid-binding Ig-like Lectin 3, SIGLEC3, Siglec-3)

Human CD33 is a 67kD sialoadhesin belonging to the Ig superfamily. It is expressed on normal myeloid cells and precursors, as well as myeloid leukemia cells, but is not present on stem cells. Antibody WM-53 is not cytotoxic, and has no detectable effect on hemopoetic cell progenitor assays.\n\nApplications:\nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: 5ug/ml\nOptimal dilutions to be determined by the researcher. \n\nFlow Cytometry Protocol: \n1. 5x10e5 cultured human HL60 cells were pre-incubated for ~5min with 20ul of 250ug/ml human Ig (to block non specific binding) after which they were incubated 45 minutes on ice with 80ul of C2385-02M at 5ug/ml. \n2. Cells were washed twice and incubated with IgG (FITC) goat x mouse , after which they were washed three times, fixed and analyzed by FACS. \n3. Cells stained positive with a mean shift of 1.22 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C2385-02M

Size

100ug

Applications

FC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.5.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

References

1. Favaloro E.J., M.C. Berndt, et al. (1987) Disease Markers 5:215-225, 2. Favaloro E.J., H. Zola, et al. (1987) British J Haematology 69:163-171, 3. Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 972-974.

Additional Info

Recognizes a common epitope on human CD33 at ~67kD