Home  >  Products  >  CD3e (T-cell Surface Glycoprotein CD3 epsilon Chain, T-cell Surface Antigen T3/Leu-4 epsilon Chain, T3E) (Biotin)
CD3e (T-cell Surface Glycoprotein CD3 epsilon Chain, T-cell Surface Antigen T3/Leu-4 epsilon Chain, T3E) (Biotin)

CD3e (T-cell Surface Glycoprotein CD3 epsilon Chain, T-cell Surface Antigen T3/Leu-4 epsilon Chain, T3E) (Biotin)

Cat no: C2254-18F

Supplier: United States Biological
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CD3e is a 20kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. This antibody is useful for in vitro blocking and activation assays, as well as apoptosis induction and in vivo T cell depletions. Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: (same/less than)0.25ug/10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2254-18F
Reactivities: Mouse
Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugates: Biotin
Size: 50ug
Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Labeled with Biotin.
P type: Mab
Purity: Purified by immunoaffinity chromatography.
References: 1. Leo, O., et al., 1987. PNAS 84:1374. 2. Kruisbeek, A.M., et al., 1991. In Current Protocols in Immunology. pp. 3.12.1-3.12.14. 3. Duke, R.C., et al., 1995. Current Protocols in Immunology. pp. 3.17.1-3.17.33. 4. Salvadori, S., et al., 1994. J. Immunol. 153:5176. 5. Payer, E., et al., 1991. J. Immunol. 146:2536. 6. Jacobs, H., et al., 1994. Eur. J. Immunol. 24:934. 7. Vossen, A.C.T.M., et al., 1995. Eur. J. Immunol. 25:1492. 8. Henrickson, M. et al., 1995. Transplantation 60:828. 9. Kinnaert, P., et al., 1996. Transpl. Int. 9:386. 10. Han, W.R., et al., 1999. Transpl. Immunol. 7:207. 11. Miescher, G.C., et al., 1989. Immunol. Lett. 23:113. 12. Terrazas, L. I., et al., 2005. Intl. J. Parasitology. 35:1349. 13. Ko, S-Y., et al., 2005. J. Immunol. 175:3309. 14. Podd, B.S., et al., 2006. J. Immunol. 176: 6532.
Additional info: Recognizes mouse CD3e.

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