CD4 (CD4 Receptor, CD4mut, T Cell Surface Antigen T4/Leu3, T Cell Surface Glycoprotein CD4)

CD4, a single chain transmembrane glycoprotein is found on a T cell subset (helper/inducer) representing 45% of peripheral blood lympocytes. It is also present on 80% of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV. Antibody to CD4 recognizes T-helper cells required for recognition of class II antigens. It reacts with 60% of peripheral blood E rosette-positive (E+) cells while showing negligible reactivity with E+ cells, monocytes, granulocytes, EBV-transformed B cell lines, and mouse splenocytes.\nCD4-positive T-cells provide helper funciton in pokeweed mitogen driven B-cell differentiation. Moreover, anti-CD4 selectively blocks the autologus mixed lymphocyte reaction (AMLR) with minimal or no effect on the allogeneic MLR (allo-MLR). Blocking of the autoreactivity occurs when either autologus B-lymphocytes or macrophages are used as stimulators. Specificity closely approximates the binding spectrum of OKT4 and Leu-3 antibodies both of which also recognize 55 to 65% of human peripheral blood T-cells.\n\nApplications:\nSuitable for use in Flow Cytometry and Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:10-1:50. Use 10ul label 10e6 cells in 100ul. \nImmunohistochemistry: Frozen\nOptimal working dilutions to be determined by researcher.\n\nRecommended Secondary Reagents: \nGoat anti-mouse IgG: HRP conjugate (non-cross reacting with rat IgG), IgG Goat anti mouse IgG: RPE (rat adsorbed)\n\nHybridoma: \nSpleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line\n\nRecommended Negative Controls: \nMouse IgG2a Negative Control\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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