CD45 otherwise known as LCA (leukocyte common antigen) is a type I transmembrane glycoprotein and member of the protein-tyrosine phosphatase (PTP) family, expressed at high levels on all nucleated cells of hematopoietic origin, and which exists in several isoforms generated by the alternative splicing. CD45 is a signal transduction protein vital for T and B cell antigen receptor-mediated activation, and is involved in many aspects of cellular processes, including growth, differentiation and oncogenic transformation. Studies have reported associations between CD45 and many cell surface antigens, including CD2, CD3, CD4, CD26 and CD90, and CD45 is used as a diagnostic histological antibody to distinguish between lymphoma and carcinoma sections.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 1:1,000-1:3,000
Optimal dilutions to be determined by the researcher.
Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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