CD45 (CD45 Antigen, B220, GP180, Leukocyte Common Antigen, LCA, L-CA, LY5, LY-5, Protein Tyrosine Phosphatase Receptor Type C, Protein Tyrosine Phosphatase Receptor Type C Polypeptide, PTPRC, T200, T200 Glycoprotein)

Applications:\nSuitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: Neat. Use 10ul of the optimal dilution to label 10e6 cells in 100ul.\nImmunohistochemistry: Frozen\nOptimal working dilutions to be determined by researcher.\n\nRecommended Secondary Reagents:\nGoat anti-mouse IgG labeled with HRP conjugate (rat absorbed)\nGoat anti mouse IgG labeled with RPE (rat adsorbed)\n\nFusion Partners: NS1 mouse myeloma line were fused with spleen cells from immunized BALB/c mice.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nAlso Available:\nC2399-01G: CD45 (Leukocyte Common Antigen, LCA, Ly-5 T200) Mab Mo xRt\nC2399-01J: CD45 (Leukocyte Common Antigen, LCA, Ly-5 T200) (Alexa Fluor(R)488) Mab Rt xMo\nC2399-08A: CD45 (Leukocyte Common Antigen, LCA, Ly-5 T200) Mab Mo xRt\nC2399-08G: CD45 (Leukocyte Common Antigen, LCA, Ly-5 T200) Mab Mo xRt\nC2399-08Y: CD45 (Leukocyte Common Antigen, LCA, Ly-5 T200) (Biotin) Mab Mo xRt\nC2399-20G: CD45 (Leukocyte Common Antigen, LCA, Ly-5 T200) (FITC) Mab Mo xRt\nC2399-20P: CD45 (Leukocyte Common Antigen, LCA, Ly-5 T200) (PE) Mab Mo xRt

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SPECIFICATIONS

Catalog Number

C2399-08G

Size

250ug

Applications

FC, IHC

Hosts

Mouse

Reactivities

Rat

Form

Supplied as a liquid with 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Sunderland, C.A., McMaster, W.R., Williams, A.F., Eur. J. Immunol. 9: 155-1592 (1979). 2. Woollett, G.R., Barclay, A.N., Puklavec, M., Williams, A.F., Eur. J. Immunol. 15: 168-173 (1985). 3. Barclay, A.N., Jackson, D.I., Willis, A.C., Williams, A.F., Embo J. 6: 1259-1264 (1987). 4. Barclay, A.N., Immunology 42: 593-600 (1981).

Additional Info

Recognizes a monomorphic determinant of the rat leucocyte common antigen (LC-A) (1). The antigen recognized is a heavily glycosylated membrane glycoprotein of MW 170,000 on thymocytes but MW 170,000-220,000 on other leucocytes (2,3). It is a useful marker for leucocytes as it has not been detected on any other cell type.