CD45 (CD45 Antigen, B220, GP180, Leukocyte Common Antigen, LCA, L-CA, LY5, LY-5, Protein Tyrosine Phosphatase Receptor Type C, Protein Tyrosine Phosphatase Receptor Type C Polypeptide, PTPRC, T200, T200 Glycoprotein) (FITC)

CD45 is a complex molecule existing in a number of isoforms. Antibodies recognizing a common epitope on all of these isoforms are termed CD45 while those recognizing only individual isoforms are termed CD45RA or CD45RO etc.\n\nApplications:\nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: Neat, 1:10. 10ul labels 10e6 cells in 100ul\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nNS1 myeloma cells with spleen cells from Balb/c mice. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

C2399-07F1

Size

100Tests

Applications

FC

Hosts

Mouse

Reactivities

Bov

Form

Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Huang, L. et al. (2010) A hierarchy of endothelial colony-forming cell activity displayed by bovine corneal endothelial cells. Invest Ophthalmol Vis Sci. 51: 3943-9. 2. Bembridge, et al. (1995) CD45RO expression on bovine T cells: relation to biological function. Immunology. 86: 537-44. 3. Lynch, E.M. et al. (2012) Effect of pre-weaning concentrate supplementation on peripheral distribution of leukocytes, functional activity of neutrophils, acute phase protein and behavioural responses of abruptly weaned and housed beef calves BMC Veterinary Research 8: 1.

Additional Info

Recognizes the bovine homolog of the human CD45 antigen and is expressed on pan leukocyte cells.