CD58 (LFA-3) (Biotin)\n

CD58, or LFA-3, is a membrane glycoprotein of 55-70kD. It occurs in two forms, one transmembrane with a cytoplasmic domain, the other form anchored in the membrane via a glycosylphosphatidylinositol tail. The complete amino acid sequence of both forms has been deduced from cDNA. It is heavily N-glycosylated. CD58 is a cell adhesion molecule which plays a critical role in facilitation of antigen specific recognition through interaction with CD2 on T lymphocytes[1]. It is a member of the immunoglobulin superfamily of molecules.\nCD58 was produced in response to erythrocytes. The functional affinity of CD58 binding to erythrocytes is 4 x 108 M-1. It reacts by immunoblotting to non-reduced erythrocyte membranes. BRIC5 is an indirect haemagglutinin. The antigen on erythrocytes is Pronase sensitive. BRIC5 inhibits T cell rosetting.\n\nApplications: \nSuitable for use in Flow Cytometry. Other applications not tested. \n\nRecommended Dilution: \nFlow Cytometry: 1:10, Use 10ul to label 10e6 cells in 100ul.\nOptimal dilution determined by the researcher\n\nRecommended Secondary Reagents: \nStreptavidin:FITC\nStreptavidin:RPE \n\nRecommended Negative Controls: \nMouse IgG2a Negative Control: Biotin\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2413-44

Size

100ug

Applications

FC

Hosts

Mouse

Reactivities

Hum

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide.\nConjugated to Biotin.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Makgoba, M.W. et al. (1989). The CD2-LFA-3 and LFA-1-ICAM pathways: relevance to T-cell recognition. Immunology Today 10: 417-422. 2. Shaw, S., Johnson, J.P., (1989) In Leucocyte Typing IV: White Cell Differentiation Antigens. Edited by Knapp, W., Dorken, B., Gilks, W.R., Rieber, E.P., Schmidt, R.E., Stein, H. and von dem Borne, A.E.G.Kr. Oxford University Press. pp 714-716. 3. Grundy, J. E., Pahal, G.S., and Akbar, A.N. (1993). Increased adherence of CD2 peripheral blood lymphocytes to CMV-infected fibroblasts is blocked by anti LFA-3 antibody. Immunology 78.

Additional Info

CD58 has a wide tissue distribution, being present on erythrocytes, platelets, monocytes, a subset of lymphocytes, bone marrow cells, epithelium and endothelial cells[2]. There are approximately 5,000 CD58 molecules on each erythrocyte. There is reduced expression of CD58 on haemopoietic cells in individuals with paroxysmal nocturnal haemoglobinuria.