CD59 (16.3A5, 1F5, 1F5 antigen, 20kD homologous restriction factor, CD59 glycoprotein, EJ16, EJ30, EL32, FLJ38134, FLJ92039, G344, HRF20, HRF-20, MACIF, MAC-inhibitory protein, MAC-IP, MEM43, MEM43 antigen, Membrane attack complex inhibition factor, Membr

Human CD59 is a GPI anchored molecule that protects cells from complement-mediated lysis (1). Studies are progressing to determine the regulatory mechanism for CD59 expression (2). \n\nApplications:\nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 5x10e5 cultured human HPB-MLT cells were incubated 45 min on ice with 80ul of anti-CD59 antibody at 5ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.04 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C2413-22H

Size

100ug

Applications

FC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1,k

References

1. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford (1995) p. 1476-1477, 2. M.H. Holguin, et al, (1996) J Immunol 157:1659-1668.

Additional Info

Recognizes human CD59.