CD69 (Activation Inducer Molecule, AIM, BLAC/P26, CLEC2C, EA1, Early Activation Antigen CD69, Early T cell Activation Antigen p60, GP32/28, Leu23, MLR-3)

CD69 is an 85kD disulphite linked homodimer, consisting of differentially glycosylated 35kD and 39kD subunits, under reducing conditions. CD69 surface expression is upregulated by T and B cell activation, and is widely expressed on hematopoetic cells, including lymphocytes, neutrophils, and eosinophils. Although not detected in resting lymphocytes, expression is rapidly upregulated within 2 hours. 2Q1673 augments PMS-induced T cell proliferation, and induces redirected lysis of Fc receptor-bearing target cells by NK cells.\n\nApplications:\nSuitable for use in Flow Cytometry and Immunohistochemistry and In vitro T cell and NK cell activation. Other applications have not been tested.\n\nRecommended Dilution:\nFlow Cytometry: (same/less than) 1ug/10e6 cells. (Acetone-fixed and frozen sections)\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2424-11

Size

500ug

Applications

FC, IHC

Reactivities

Mouse

Form

Supplied as a liquid in borate buffered saline, pH 8, before the addition of glycerol to 40%. No preservatives added.

P Type

Mab

Purity

Purified by affinity chromatography.

Isotype

IgG,l

Additional Info

Recognizes mouse CD69 or Very Early Activation Antigen (VEA). A 35kD disulphide-linked dimer, CD69 surface expression is upregulated by T and B cell activation. CD69 is also expressed by a small subset of lymphocytes in the thymus, spleen and lymph nodes.